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小鼠溴脫氧核苷尿嘧啶酶免試劑盒,(BrdU)ELISA檢測試劑盒 ,英文名:BrdU ELISA Kit ,英文縮寫:BrdU ,常見試劑盒規格:96T/48T,產品別名:小鼠溴脫氧核苷尿嘧啶ELISA檢測試劑盒,BrdU 檢測試劑盒, BrdU 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠溴脫氧核苷尿嘧啶酶免試劑盒,(BrdU)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
腫瘤壞死因子配體超家族成員12(TNFSF12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12)48T/96TY16635Homo sapiens (Human)
蛋白磷酸酶1調節因子亞基1B(PPP1R1B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B)48T/96TY16636Homo sapiens (Human)
法尼基二磷酸合酶(FDPS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Farnesyl Diphosphate Synthase (FDPS)48T/96TY16637Rattus norvegicus (Rat)
細胞分裂周期因子25(CDC25)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cell Division Cycle Protein 25 (CDC25)48T/96TY16638Homo sapiens (Human)
細胞分裂周期因子25(CDC25)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cell Division Cycle Protein 25 (CDC25)48T/96TY16639Mus musculus (Mouse)
白介素11受體α(IL11Rα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 11 Receptor Alpha (IL11Ra)48T/96TY16640Homo sapiens (Human)
白介素11受體α(IL11Rα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 11 Receptor Alpha (IL11Ra)48T/96TY16641Mus musculus (Mouse)
白介素11受體α(IL11Rα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 11 Receptor Alpha (IL11Ra)48T/96TY16642Rattus norvegicus (Rat)
PHD指蛋白8(PHF8)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for PHD Finger Protein 8 (PHF8)48T/96TY16643Mus musculus (Mouse)
異戊烯半胱氨酸氧化酶1(PCYOX1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prenylcysteine Oxidase 1 (PCYOX1)48T/96TY16644Mus musculus (Mouse)
母系胚胎亮氨酸拉鏈蛋白激酶(MELK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Maternal Embryonic Leucine Zipper Kinase (MELK)48T/96TY16645Homo sapiens (Human)
母系胚胎亮氨酸拉鏈蛋白激酶(MELK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Maternal Embryonic Leucine Zipper Kinase (MELK)48T/96TY16646Mus musculus (Mouse)
母系胚胎亮氨酸拉鏈蛋白激酶(MELK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Maternal Embryonic Leucine Zipper Kinase (MELK)48T/96TY16647Rattus norvegicus (Rat)
甲型肝炎病毒細胞受體2(HAVCR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hepatitis A Virus Cellular Receptor 2 (HAVCR2)48T/96TY16648Homo sapiens (Human)
RAS癌基因家族成員RAB1A(RAB1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for RAB1A, Member RAS Oncogene Family (RAB1A)48T/96TY16649Homo sapiens (Human)
ISL LIM同源框蛋白1(ISL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for ISL LIM Homeobox Protein 1 (ISL1)48T/96TY16650Homo sapiens (Human)
Slingshot同源物1(SSH1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Slingshot Homolog 1 (SSH1)48T/96TY16651Homo sapiens (Human)
雙特異性酪氨酸磷酸化調節激酶1A(DYRK1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A)48T/96TY16652Homo sapiens (Human)
Slingshot同源物3(SSH3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Slingshot Homolog 3 (SSH3)48T/96TY16653Homo sapiens (Human)
Slingshot同源物2(SSH2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Slingshot Homolog 2 (SSH2)48T/96TY16654Homo sapiens (Human)
UDP葡萄糖醛酸轉移酶2家族多肽B7(UGT2B7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7)48T/96TY16655Rattus norvegicus (Rat)
復制蛋白A1(RPA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Replication Protein A1 (RPA1)48T/96TY16656Rattus norvegicus (Rat)
Discs大同源物3(DLG3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Discs, Large Homolog 3 (DLG3)48T/96TY16657Homo sapiens (Human)
賴氨酸特異性去甲基化酶4A(KDM4A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lysine Specific Demethylase 4A (KDM4A)48T/96TY16658Homo sapiens (Human)
復制蛋白A1(RPA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Replication Protein A1 (RPA1)48T/96TY16659Homo sapiens (Human)
蛋氨酸腺苷轉移酶Ⅰα(MAT1α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Methionine Adenosyltransferase I Alpha (MAT1a)48T/96TY16660Rattus norvegicus (Rat)
興奮性氨基酸轉運蛋白5(EAAT5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Excitatory Amino Acid Transporter 5 (EAAT5)48T/96TY16661Homo sapiens (Human)
興奮性氨基酸轉運蛋白4(EAAT4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Excitatory Amino Acid Transporter 4 (EAAT4)48T/96TY16662Homo sapiens (Human)
色胺酸羥化酶1(TPH1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tryptophan Hydroxylase 1 (TPH1)48T/96TY16663Mus musculus (Mouse)
含TCP1伴侶蛋白亞基2(CCT2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chaperonin Containing TCP1, Subunit 2 (CCT2)48T/96TY16664Homo sapiens (Human)
含TCP1伴侶蛋白亞基2(CCT2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chaperonin Containing TCP1, Subunit 2 (CCT2)48T/96TY16665Mus musculus (Mouse)
卷曲域錨蛋白重復序列??葡萄膜自身抗原(UACA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA)48T/96TY16666Homo sapiens (Human)
節律晝夜蛋白2(PER2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Period Circadian Protein 2 (PER2)48T/96TY16667Rattus norvegicus (Rat)
CD27結合蛋白(CD27BP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for CD27 Binding Protein (CD27BP)48T/96TY16668Homo sapiens (Human)
Zeste 12同源物1抑制因子2(SUZ12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Suppressor Of Zeste 12 Homolog (SUZ12)48T/96TY16669Homo sapiens (Human)
YY1轉錄因子(YY1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for YY1 Transcription Factor (YY1)48T/96TY16670Homo sapiens (Human)
硬脂酰輔酶A去飽和酶(SCD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Stearoyl Coenzyme A Desaturase (SCD)48T/96TY16671Rattus norvegicus (Rat)
核糖體蛋白L13A(RPL13A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ribosomal Protein L13A (RPL13A)48T/96TY16672Homo sapiens (Human)
活性氧物種調節因子1(ROMO1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Reactive Oxygen Species Modulator 1 (ROMO1)48T/96TY16673Homo sapiens (Human)
腦蛋白44樣蛋白(BRP44L)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Brain Protein 44 Like Protein(BRP44L)48T/96TY16674Homo sapiens (Human)
γ-分泌激活蛋白(γSAP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gamma-Secretase Activating Protein (gSAP)48T/96TY16675Rattus norvegicus (Rat)
淀粉樣蛋白β前體樣蛋白1(APLP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Amyloid Beta Precursor Like Protein 1 (APLP1)48T/96TY16676Mus musculus (Mouse)
免疫球蛋白G2α(IgG2α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Immunoglobulin G2a (IgG2a)48T/96TY16677Mus musculus (Mouse)
淀粉樣蛋白β前體樣蛋白1(APLP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Amyloid Beta Precursor Like Protein 1 (APLP1)48T/96TY16678Homo sapiens (Human)
沉默調節蛋白6(SIRT6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sirtuin 6 (SIRT6)48T/96TY16679Mus musculus (Mouse)
N-甲基-D-天氡氨酸離子能谷氨酸受體2D(GRIN2D)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D)48T/96TY16680Homo sapiens (Human)
硬脂酰輔酶A去飽和酶(SCD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Stearoyl Coenzyme A Desaturase (SCD)48T/96TY16681Mus musculus (Mouse)
17-β-羥基類固醇脫氫酶10(HSD17β10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10)48T/96TY16682Mus musculus (Mouse)
肌鈣蛋白I(TNI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin I (TNI)48T/96TY16683Homo sapiens (Human)
周期素依賴性激酶5(CDK5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 5 (CDK5)48T/96TY16684Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。