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小鼠內臟脂肪特異性絲氨酸蛋白酶抑制劑酶免試劑盒,(vaspin)ELISA檢測試劑盒 ,英文名:vaspin ELISA Kit ,英文縮寫:vaspin ,常見試劑盒規格:96T/48T,產品別名:小鼠內臟脂肪特異性絲氨酸蛋白酶抑制劑ELISA檢測試劑盒,vaspin 檢測試劑盒, vaspin 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠內臟脂肪特異性絲氨酸蛋白酶抑制劑酶免試劑盒,(vaspin)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
V-Ki-Ras2 Kirsten大鼠肉瘤病毒癌基因同源物(KRAS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS)48T/96TY16585Homo sapiens (Human)
白介素18結合蛋白(IL18BP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 18 Binding Protein (IL18BP)48T/96TY16586Homo sapiens (Human)
早期內體抗原1(EEA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Early Endosome Antigen 1 (EEA1)48T/96TY16587Homo sapiens (Human)
多萜醇磷酸-N-乙酰氨基葡萄糖磷酸轉移酶1(DPAGT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1)48T/96TY16588Homo sapiens (Human)
DNA損傷誘導轉錄因子3(DDIT3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for DNA Damage Inducible Transcript 3 (DDIT3)48T/96TY16589Homo sapiens (Human)
H2A組蛋白家族成員V(H2AFV)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for H2A Histone Family, Member V (H2AFV)48T/96TY16590Homo sapiens (Human)
B-細胞κ-輕肽基因增強子抑制因子激酶β(IκBKβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb)48T/96TY16591Homo sapiens (Human)
Snail同源物1(SNAI1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Snail Homolog 1 (SNAI1)48T/96TY16592Homo sapiens (Human)
神經調節素4(NRG4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuregulin 4 (NRG4)48T/96TY16593Mus musculus (Mouse)
含Kazal型絲氨酸蛋白酶抑制劑域蛋白1(KAZALD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1)48T/96TY16594Homo sapiens (Human)
棘皮動物微管關聯蛋白樣蛋白2(EML2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Echinoderm Microtubule Associated Protein Like Protein 2 (EML2)48T/96TY16595Rattus norvegicus (Rat)
血管緊張素1-7(Ang1-7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Angiotensin 1-7 (Ang1-7)48T/96TY16596Rattus norvegicus (Rat)
血管緊張素Ⅱ(ANGⅡ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Angiotensin II (AngII)48T/96TY16597Equus caballus; Equine (Horse)
干擾素γ(IFNγ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY16598Equus caballus; Equine (Horse)
吻素受體(KISS1R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kisspeptin Receptor (KISS1R)48T/96TY16599Mus musculus (Mouse)
吻素受體(KISS1R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kisspeptin Receptor (KISS1R)48T/96TY16600Rattus norvegicus (Rat)
G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER)48T/96TY16601Mus musculus (Mouse)
嘌呤能受體P2Y12(P2RY12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12)48T/96TY16602Homo sapiens (Human)
微管關聯蛋白1輕鏈3α(MAP1LC3α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a)48T/96TY16603Homo sapiens (Human)
類固醇合成急性調節蛋白(STAR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Steroidogenic Acute Regulatory Protein (STAR)48T/96TY16604Homo sapiens (Human)
G蛋白偶聯受體78(GPR78)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for G Protein Coupled Receptor 78 (GPR78)48T/96TY16605Homo sapiens (Human)
抗繆勒管激素(AMH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Anti Mullerian Hormone (AMH)48T/96TY16606Bos taurus; Bovine (Cattle)
肌鈣蛋白I(TNI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin I (TNI)48T/96TY16607General
α-內啡肽(αEP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-Endorphin (aEP)48T/96TY16608Mus musculus (Mouse)
抑制素B(INHB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Inhibin B (INHB)48T/96TY16609Bos taurus; Bovine (Cattle)
生長激素受體(GHR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Hormone Receptor (GHR)48T/96TY16610Mus musculus (Mouse)
玻連蛋白(VTN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vitronectin (VTN)48T/96TY16611Rattus norvegicus (Rat)
70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A)48T/96TY16612Mus musculus (Mouse)
軟骨糖蛋白39(GP39)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycoprotein 39, Cartilage (GP39)48T/96TY16613Rattus norvegicus (Rat)
胸腺素β4(Tβ4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thymosin Beta 4 (Tb4)48T/96TY16614Homo sapiens (Human)
核因子κB2(NFκB2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nuclear Factor Kappa B2 (NFkB2)48T/96TY16615Homo sapiens (Human)
內質網核心信號1(ERN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1)48T/96TY16616Homo sapiens (Human)
吻素1(KISS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kisspeptin 1 (KISS1)48T/96TY16617Mus musculus (Mouse)
微精蛋白β(MSMβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Microseminoprotein Beta (MSMb)48T/96TY16618Mus musculus (Mouse)
跨膜絲氨酸蛋白酶2(TMPRSS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transmembrane Protease, Serine 2 (TMPRSS2)48T/96TY16619Mus musculus (Mouse)
腫廇蛋白P63(TP63)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Protein P63 (TP63)48T/96TY16620Mus musculus (Mouse)
腎酶(RNLS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Renalase (RNLS)48T/96TY16621Rattus norvegicus (Rat)
γ-內啡肽(γEP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gamma-Endorphin (gEP)48T/96TY16622Mus musculus (Mouse)
低密度脂蛋白受體相關蛋白5(LRP5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Low Density Lipoprotein Receptor Related Protein 5 (LRP5)48T/96TY16623Rattus norvegicus (Rat)
抗原肽轉運蛋白1(TAP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Antigen Peptide Transporter 1 (TAP1)48T/96TY16624Homo sapiens (Human)
心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)48T/96TY16625Oryctolagus cuniculus (Rabbit)
Y-染色體胸腺素β4(Tβ4Y)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thymosin Beta 4, Y-Chromosomal (Tb4Y)48T/96TY16626Homo sapiens (Human)
腎臟腦蛋白(KIBRA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kidney And Brain Protein (KIBRA)48T/96TY16627Mus musculus (Mouse)
細胞色素C氧化酶亞基Ⅳ亞型1(COX4I1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1)48T/96TY16628Homo sapiens (Human)
細胞色素b-245β肽(CYBβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytochrome b-245 Beta Polypeptide (CYBb)48T/96TY16629Homo sapiens (Human)
左右決定因子2(LEFTY2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Left/Right Determination Factor 2 (LEFTY2)48T/96TY16630Mus musculus (Mouse)
酪蛋白激酶1δ(CSNK1δ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Casein Kinase 1 Delta (CSNK1d)48T/96TY16631Homo sapiens (Human)
Dickkopf相關蛋白3(DKK3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dickkopf Related Protein 3 (DKK3)48T/96T Y16632Rattus norvegicus (Rat)
蛋白二硫化物異構酶A4(PDIA4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Disulfide Isomerase A4 (PDIA4)48T/96TY16633Mus musculus (Mouse)
ⅡA組磷脂酶A2(PLA2G2A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phospholipase A2, Group IIA (PLA2G2A)48T/96TY16634Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。