服務熱線
021-54479081
021-54461587
產品展示PRODUCTS
本產品僅用于科研,不用于臨床
小鼠乙型肝炎核心抗體酶免試劑盒,(HBcAb)ELISA檢測試劑盒 ,英文名:HBcAb ELISA Kit ,英文縮寫:HBcAb ,常見試劑盒規格:96T/48T,產品別名:小鼠乙型肝炎核心抗體ELISA檢測試劑盒,HBcAb 檢測試劑盒, HBcAb 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠乙型肝炎核心抗體酶免試劑盒,(HBcAb)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
糖原蛋白1(GYG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycogenin 1 (GYG1)48T/96TY15885Homo sapiens (Human)
樣糖基轉移酶(LARGE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Like Glycosyltransferase (LARGE)48T/96TY15886Homo sapiens (Human)
鳥苷酸激酶1(GUK1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Guanylate Kinase 1 (GUK1)48T/96TY15887Homo sapiens (Human)
Goosecoid 2蛋白(GSC2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Goosecoid 2 (GSC2)48T/96TY15888Homo sapiens (Human)
Goosecoid蛋白(GSC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Goosecoid (GSC)48T/96TY15889Homo sapiens (Human)
橋尾蛋白(GPHN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gephyrin (GPHN)48T/96TY15890Homo sapiens (Human)
糖蛋白A33(GPA33)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycoprotein A33 (GPA33)48T/96TY15891Homo sapiens (Human)
高爾基磷蛋白3(GOLPH3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgi Phosphoprotein 3 (GOLPH3)48T/96TY15892Homo sapiens (Human)
高爾基體蛋白B1(GOLGB1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin B1 (GOLGB1)48T/96TY15893Homo sapiens (Human)
高爾基體蛋白A1(GOLGA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin A1 (GOLGA1)48T/96TY15894Homo sapiens (Human)
高爾基體蛋白A2(GOLGA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin A2 (GOLGA2)48T/96TY15895Homo sapiens (Human)
高爾基體蛋白A3(GOLGA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin A3 (GOLGA3)48T/96TY15896Homo sapiens (Human)
高爾基體蛋白A4(GOLGA4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin A4 (GOLGA4)48T/96TY15897Homo sapiens (Human)
高爾基體蛋白A5(GOLGA5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin A5 (GOLGA5)48T/96TY15898Homo sapiens (Human)
高爾基體蛋白A6(GOLGA6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin A6 (GOLGA6)48T/96TY15899Homo sapiens (Human)
高爾基體蛋白A7(GOLGA7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgin A7 (GOLGA7)48T/96TY15900Homo sapiens (Human)
甘油磷酸-O-酰基轉移酶(GNPAT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glyceronephosphate-O-Acyltransferase (GNPAT)48T/96TY15901Homo sapiens (Human)
甘胺酸-N-酰基轉移酶(GLYAT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycine-N-Acyltransferase (GLYAT)48T/96TY15902Homo sapiens (Human)
谷氨酸脫氫酶2(GLUD2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutamate Dehydrogenase 2 (GLUD2)48T/96TY15903Homo sapiens (Human)
谷氨酰胺酶2(GLS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutaminase 2 (GLS2)48T/96TY15904Homo sapiens (Human)
神經膠質蛋白(GLDN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gliomedin (GLDN)48T/96TY15905Homo sapiens (Human)
甘油激酶2(GK2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycerol Kinase 2 (GK2)48T/96TY15906Homo sapiens (Human)
甘油激酶(GK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycerol Kinase (GK)48T/96TY15907Homo sapiens (Human)
配子發育蛋白(GGN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gametogenetin (GGN)48T/96TY15908Homo sapiens (Human)
γ-谷氨酰環化轉移酶(γGCT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gamma-Glutamylcyclotransferase (gGCT)48T/96TY15909Homo sapiens (Human)
鳥嘌呤脫氨酶(GDA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Guanine Deaminase (GDA)48T/96TY15910Homo sapiens (Human)
GTP環化水解酶1(GCH1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for GTP Cyclohydrolase 1 (GCH1)48T/96TY15911Homo sapiens (Human)
磷酸核糖甘氨酰胺轉甲酰基酶(GART)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phosphoribosylglycinamide Formyltransferase (GART)48T/96TY15912Homo sapiens (Human)
半乳糖激酶2(GALK2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactokinase 2 (GALK2)48T/96TY15913Homo sapiens (Human)
半乳糖激酶1(GALK1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactokinase 1 (GALK1)48T/96TY15914Homo sapiens (Human)
半乳糖變旋酶(GALM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactose Mutarotase (GALM)48T/96TY15915Homo sapiens (Human)
UDP半乳糖-4-差向異構酶(GALE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for UDP Galactose-4-Epimerase (GALE)48T/96TY15916Homo sapiens (Human)
半乳糖-3-O-磺基轉移酶3(GAL3ST3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactose-3-O-Sulfotransferase 3 (GAL3ST3)48T/96TY15917Homo sapiens (Human)
半乳糖-3-O-磺基轉移酶4(GAL3ST4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactose-3-O-Sulfotransferase 4 (GAL3ST4)48T/96TY15918Homo sapiens (Human)
半乳糖-3-O-磺基轉移酶2(GAL3ST2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactose-3-O-Sulfotransferase 2 (GAL3ST2)48T/96TY15919Homo sapiens (Human)
半乳糖-3-O-磺基轉移酶1(GAL3ST1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactose-3-O-Sulfotransferase 1 (GAL3ST1)48T/96TY15920Homo sapiens (Human)
G抗原1(GAGE1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for G Antigen 1 (GAGE1)48T/96TY15921Homo sapiens (Human)
巖藻糖激酶(FUK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fucokinase (FUK)48T/96TY15922Homo sapiens (Human)
胺甲基四氫葉酸環化脫氫酶(FTCD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Formiminotransferase Cyclodeaminase (FTCD)48T/96TY15923Homo sapiens (Human)
法尼基轉移酶β(FNTβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Farnesyltransferase Beta (FNTb)48T/96TY15924Homo sapiens (Human)
果糖胺-3-激酶(FN3K)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fructosamine-3-Kinase (FN3K)48T/96TY15925Homo sapiens (Human)
形成素2(FMN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Formin 2 (FMN2)48T/96TY15926Homo sapiens (Human)
形成素1(FMN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Formin 1 (FMN1)48T/96TY15927Homo sapiens (Human)
Fission 1蛋白(FIS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fission 1 (FIS1)48T/96TY15928Homo sapiens (Human)
Fidgetin蛋白(FIGN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fidgetin (FIGN)48T/96TY15929Homo sapiens (Human)
延胡索二酰乙酰乙酸水解酶(FAH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fumarylacetoacetate Hydrolase (FAH)48T/96TY15930Homo sapiens (Human)
Exophilin 5蛋白(EXPH5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Exophilin 5 (EXPH5)48T/96TY15931Homo sapiens (Human)
外切酶體成分1(EXOSC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Exosome Component 1 (EXOSC1)48T/96TY15932Homo sapiens (Human)
Ets變異體3(ETV3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ets Variant 3 (ETV3)48T/96TY15933Homo sapiens (Human)
Ets變異體2(ETV2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ets Variant 2 (ETV2)48T/96TY15934Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。