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小鼠嗜酸性粒細胞陽離子蛋白酶免試劑盒,(ECP)ELISA檢測試劑盒 ,英文名:ECP ELISA Kit ,英文縮寫:ECP ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠嗜酸性粒細胞陽離子蛋白ELISA檢測試劑盒,ECP 檢測試劑盒, ECP 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠嗜酸性粒細胞陽離子蛋白酶免試劑盒,(ECP)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結(jié)合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
腺嘌呤磷酸核糖轉(zhuǎn)移酶(APRT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenine Phosphoribosyltransferase (APRT)48T/96TY13185Homo sapiens (Human)
脫氧胞苷激酶(DCK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Deoxycytidine Kinase (DCK)48T/96TY13186Homo sapiens (Human)
可溶性胸苷激酶1(TK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymidine Kinase 1, Soluble (TK1)48T/96TY13187Homo sapiens (Human)
氫/鉀離子交換ATP酶α肽(ATP4α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)48T/96TY13188Mus musculus (Mouse)
衰老關鍵蛋白3(FBLN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibulin 3 (FBLN3)48T/96TY13189Homo sapiens (Human)
胰脂肪酶(PL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Pancreatic (PL)48T/96TY13190Rattus norvegicus (Rat)
核纖層蛋白A/C(LMNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lamin A/C (LMNA)48T/96TY13191Homo sapiens (Human)
髓分化因子88(MyD88)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myeloid Differentiation Factor 88 (MyD88)48T/96TY13192Mus musculus (Mouse)
法尼酯X受體(FXR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Farnesoid X Receptor (FXR)48T/96TY13193Mus musculus (Mouse)
骨骼肌快肌肌鈣蛋白I(TNNI2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin I Type 2, Fast Skeletal (TNNI2)48T/96TY13194Homo sapiens (Human)
趨化因子C-X-C-基元受體7(CXCR7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X-C-Motif Receptor 7 (CXCR7)48T/96TY13195Homo sapiens (Human)
磷脂酶A2受體1(PLA2R1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2 Receptor 1 (PLA2R1)48T/96TY13196Homo sapiens (Human)
封閉蛋白11(CLDN11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Claudin 11 (CLDN11)48T/96TY13197Homo sapiens (Human)
前列腺六跨膜表皮抗原2(STEAP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2)48T/96TY13198Homo sapiens (Human)
DNA甲基轉(zhuǎn)移酶1(DNMT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for DNA Methyltransferase 1 (DNMT1)48T/96TY13199Homo sapiens (Human)
DNA酶Ⅰ樣蛋白3(DNASE1L3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Deoxyribonuclease I Like Protein 3 (DNASE1L3)48T/96TY13200Mus musculus (Mouse)
死亡關聯(lián)蛋白激酶1(DAPK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Death Associated Protein Kinase 1 (DAPK1)48T/96TY13201Homo sapiens (Human)
原鈣黏素β16(PCDHβ16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protocadherin Beta 16 (PCDHb16)48T/96TY13202Homo sapiens (Human)
多聚ADP核糖聚合酶4(PARP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Poly ADP Ribose Polymerase 4 (PARP4)48T/96TY13203Homo sapiens (Human)
鈣離子轉(zhuǎn)運ATP酶A2(ATP2A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)48T/96TY13204Mus musculus (Mouse)
中間α-球蛋白抑制因子H3(ITIH3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inter Alpha-Globulin Inhibitor H3 (ITIH3)48T/96TY13205Homo sapiens (Human)
成纖維細胞生長因子15(FGF15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 15 (FGF15)48T/96TY13206Rattus norvegicus (Rat)
叉頭框蛋白O3(FOXO3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein O3 (FOXO3)48T/96TY13207Mus musculus (Mouse)
軟骨關聯(lián)蛋白(CRTAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cartilage Associated Protein (CRTAP)48T/96TY13208Homo sapiens (Human)
扭轉(zhuǎn)原腸胚形成同源物1(TWSG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Twisted Gastrulation Protein Homolog 1 (TWSG1)48T/96TY13209Homo sapiens (Human)
端粒酶逆轉(zhuǎn)錄酶(TERT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for omerase Reverse Transcriptase (TERT)48T/96TY13210Homo sapiens (Human)
BMP結(jié)合內(nèi)皮調(diào)節(jié)因子(BMPER)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for BMP Binding Endothelial Regulator (BMPER)48T/96TY13211Homo sapiens (Human)
基質(zhì)金屬蛋白酶14(MMP14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 14 (MMP14)48T/96TY13212Mus musculus (Mouse)
BMP結(jié)合內(nèi)皮調(diào)節(jié)因子(BMPER)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for BMP Binding Endothelial Regulator (BMPER)48T/96TY13213Mus musculus (Mouse)
鈣磷蛋白(CAPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcyphosine (CAPS)48T/96TY13214Homo sapiens (Human)
奇異不良素(DYSF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dysferlin (DYSF)48T/96TY13215Homo sapiens (Human)
補體成分1q子成分樣蛋白1(C1qL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 1, Q Subcomponent Like Protein 1 (C1qL1)48T/96TY13216Homo sapiens (Human)
補體成分1q子成分C(C1qC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 1, Q Subcomponent C (C1qC)48T/96TY13217Homo sapiens (Human)
斯鈣素2(STC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stanniocalcin 2 (STC2)48T/96TY13218Homo sapiens (Human)
鈣粘蛋白EGF LAG七經(jīng)G-型受體2(CELSR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2)48T/96TY13219Homo sapiens (Human)
氨基己糖苷酶Aα(HEXα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hexosaminidase A Alpha (HEXa)48T/96TY13220Homo sapiens (Human)
抑制素βB(INHβB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin Beta B (INHbB)48T/96TY13221Mus musculus (Mouse)
羧肽酶A3(CPA3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carboxypeptidase A3, Mast Cell (CPA3)48T/96TY13222Homo sapiens (Human)
Slit同源物1(Slit1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Slit Homolog 1 (Slit1)48T/96TY13223Rattus norvegicus (Rat)
存活素(Surv)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Survivin (Surv)48T/96TY13224Mus musculus (Mouse)
單核細胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY13225Canis familiaris; Canine (Dog)
ATP結(jié)合盒轉(zhuǎn)運蛋白G2(ABCG2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATP Binding Cassette Transporter G2 (ABCG2)48T/96TY13226Mus musculus (Mouse)
骨骼肌特異性受體酪氨酸激酶(MUSK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)48T/96TY13227Mus musculus (Mouse)
黑素瘤抑制性活性蛋白1(MIA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melanoma Inhibitory Activity Protein 1 (MIA1)48T/96TY13228Rattus norvegicus (Rat)
FK506結(jié)合蛋白樣蛋白(FKBPL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for FK506 Binding Protein Like Protein (FKBPL)48T/96TY13229Homo sapiens (Human)
嗜乳脂蛋白亞家族1成員A1(BTN1A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Butyrophilin Subfamily 1, Member A1 (BTN1A1)48T/96TY13230Homo sapiens (Human)
腱調(diào)蛋白(TNMD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tenomodulin (TNMD)48T/96TY13231Homo sapiens (Human)
碳酸酐酶Ⅲ(CA3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase III, Muscle Specific (CA3)48T/96TY13232Homo sapiens (Human)
補體成分1q子成分A(C1qA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 1, Q Subcomponent A (C1qA)48T/96TY13233Homo sapiens (Human)
17-β-羥基類固醇脫氫酶14(HSD17β14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14)48T/96TY13234Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。