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小鼠小扁豆素結合型甲胎蛋白/甲胎蛋白異質體3酶免試劑盒,(AFP-L3)ELISA檢測試劑盒 ,英文名:AFP-L3 ELISA Kit ,英文縮寫:AFP-L3 ,常見試劑盒規格:96T/48T,產品別名:小鼠小扁豆素結合型甲胎蛋白/甲胎蛋白異質體3ELISA檢測試劑盒,AFP-L3 檢測試劑盒, AFP-L3 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠小扁豆素結合型甲胎蛋白/甲胎蛋白異質體3酶免試劑盒,(AFP-L3)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
抗凝血酶(AT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Antithrombin (AT)48T/96TY12035Mus musculus (Mouse)
趨化因子C-C-基元受體2(CCR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Receptor 2 (CCR2)48T/96TY12036Homo sapiens (Human)
雌激素受體β(ERβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Estrogen Receptor Beta (ERb)48T/96TY12037Rattus norvegicus (Rat)
雌激素受體α(ERα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Estrogen Receptor Alpha (ERa)48T/96TY12038Mus musculus (Mouse)
雄激素受體(AR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Androgen Receptor (AR)48T/96TY12039Mus musculus (Mouse)
芳香烴受體(AhR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aryl Hydrocarbon Receptor (AhR)48T/96TY12040Mus musculus (Mouse)
核因子κB受體激活因子(RANκ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B (RANk)48T/96TY12041Homo sapiens (Human)
胃泌素(GT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gastrin (GT)48T/96TY12042Canis familiaris; Canine (Dog)
極低密度脂蛋白(VLDL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Very Low Density Lipoprotein (VLDL)48T/96TY12043Homo sapiens (Human)
成纖維細胞生長因子4(FGF4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 4 (FGF4)48T/96TY12044Bos taurus; Bovine (Cattle)
成纖維細胞生長因子4(FGF4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 4 (FGF4)48T/96TY12045Chicken (Gallus)
成纖維細胞生長因子6(FGF6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 6 (FGF6)48T/96TY12046Danio rerio (Zebrafish)
成纖維細胞生長因子9(FGF9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 9 (FGF9)48T/96TY12047Chicken (Gallus)
成纖維細胞生長因子9(FGF9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 9 (FGF9)48T/96TY12048Sus scrofa; Porcine (Pig)
干擾素α(IFNα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY12049Bos taurus; Bovine (Cattle)
干擾素α(IFNα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY12050Equus caballus; Equine (Horse)
載脂蛋白C1(APOC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein C1 (APOC1)48T/96TY12051Bos taurus; Bovine (Cattle)
氨乙酰丙酸δ脫水酶(ALAD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aminolevulinate Delta Dehydratase (ALAD)48T/96TY12052Homo sapiens (Human)
亞鐵螯合酶(FECH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ferrochelatase (FECH)48T/96TY12053Rattus norvegicus (Rat)
70kDa熱休克蛋白5(HSPA5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5)48T/96TY12054Homo sapiens (Human)
成纖維細胞生長因子3(FGF3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 3 (FGF3)48T/96TY12055Homo sapiens (Human)
YY肽(PYY)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Peptide YY (PYY)48T/96TY12056Rattus norvegicus (Rat)
Ⅰ類主要組織相容性復合體C(MHCC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Major Histocompatibility Complex Class I C (MHCC)48T/96TY12057Homo sapiens (Human)
胰島素樣生長因子結合蛋白5(IGFBP5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 5 (IGFBP5)48T/96TY12058Bos taurus; Bovine (Cattle)
轉甲狀腺素蛋白(TTR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transthyretin (TTR)48T/96TY12059Bos taurus; Bovine (Cattle)
突觸核蛋白α(SNCα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Synuclein Alpha (SNCa)48T/96TY12060Mus musculus (Mouse)
滲透性糖蛋白(Pgp)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Permeability Glycoprotein (Pgp)48T/96TY12061Mus musculus (Mouse)
Prominin 1蛋白(PROM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prominin 1 (PROM1)48T/96TY12062Homo sapiens (Human)
補體成分5(C5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Complement Component 5 (C5)48T/96TY12063Mus musculus (Mouse)
硬骨素(SOST)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sclerostin (SOST)48T/96TY12064Rattus norvegicus (Rat)
過氧化氫酶(CAT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Catalase (CAT)48T/96TY12065Rattus norvegicus (Rat)
血漿谷胱甘肽過氧化物酶(GPX3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutathione Peroxidase 3, Plasma (GPX3)48T/96TY12066Rattus norvegicus (Rat)
乙酰膽堿酯酶(AchE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Acetylcholinesterase (AchE)48T/96TY12067Rattus norvegicus (Rat)
Ⅹ型膠原(COL10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type X (COL10)48T/96TY12068Mus musculus (Mouse)
維生素B1(VB1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vitamin B1 (VB1)48T/96TY12069General
硒蛋白P1(SEPP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Selenoprotein P1, Plasma (SEPP1)48T/96TY12070Mus musculus (Mouse)
硒蛋白P1(SEPP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Selenoprotein P1, Plasma (SEPP1)48T/96TY12071Rattus norvegicus (Rat)
載脂蛋白B(APOB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein B (APOB)48T/96TY12072Homo sapiens (Human)
組織蛋白酶S(CTSS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cathepsin S (CTSS)48T/96TY12073Mus musculus (Mouse)
血紅素結合蛋白(HPX)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hemopexin (HPX)48T/96TY12074Bos taurus; Bovine (Cattle)
補體成分4(C4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Complement Component 4 (C4)48T/96TY12075Mus musculus (Mouse)
血管活性腸肽(VIP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vasoactive Intestinal Peptide (VIP)48T/96TY12076Bos taurus; Bovine (Cattle)
內皮抑素(ES)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endostatin (ES)48T/96TY12077Rattus norvegicus (Rat)
血管抑素(ANG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Angiostatin (ANG)48T/96TY12078Rattus norvegicus (Rat)
肌肉生長抑制素(MSTN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myostatin (MSTN)48T/96TY12079Rattus norvegicus (Rat)
免疫球蛋白G2(IgG2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Immunoglobulin G2 (IgG2)48T/96TY12080Cavia (Guinea pig )
末端補體復合體C5b-9(C5b-9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)48T/96T,(AFP-L3)ELISA檢測試劑盒Y12081Sus scrofa; Porcine (Pig)
破骨細胞關聯受體(OSCAR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Osteoclast Associated Receptor (OSCAR)48T/96TY12082Homo sapiens (Human)
高遷移率族蛋白1(HMG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for High Mobility Group Protein 1 (HMG1)48T/96TY12083Canis familiaris; Canine (Dog)
高遷移率族蛋白1(HMG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for High Mobility Group Protein 1 (HMG1)48T/96TY12084Sus scrofa; Porcine (Pig)
各種類型ELISA測定時,,(AFP-L3)ELISA檢測試劑盒一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。