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小鼠IgM ELISA檢測試劑盒,英文名:小鼠IgM ELISA試劑盒,英文縮寫:小鼠IgM ELISA試劑盒,常見試劑盒規格:96T/48T,產品別名:小鼠IgM ELISA試劑盒小鼠IgMELISA試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠IgM ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
白介素1α(IL1α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11735Canis familiaris; Canine (Dog)
白介素1α(IL1α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11736Capra hircus; Caprine (Goat)
白介素1α(IL1α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11737Equus caballus; Equine (Horse)
白介素1α(IL1α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11738Cavia (Guinea pig )
白介素1α(IL1α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY11739Oryctolagus cuniculus (Rabbit)
防御素β2(DEFβ2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Beta 2 (DEFb2)48T/96TY11740Capra hircus; Caprine (Goat)
防御素β2(DEFβ2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Beta 2 (DEFb2)48T/96TY11741Equus caballus; Equine (Horse)
防御素β2(DEFβ2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Beta 2 (DEFb2)48T/96TY11742Mus musculus (Mouse)
白介素2(IL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 2 (IL2)48T/96TY11743Equus caballus; Equine (Horse)
免疫球蛋白G1(IgG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Immunoglobulin G1 (IgG1)48T/96TY11744Mus musculus (Mouse)
基質金屬蛋白酶12(MMP12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 12 (MMP12)48T/96TY11745Rattus norvegicus (Rat)
半乳糖苷酶β(GLβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY11746Bos taurus; Bovine (Cattle)
半乳糖苷酶β(GLβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY11747Canis familiaris; Canine (Dog)
半乳糖苷酶β(GLβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY11748Danio rerio (Zebrafish)
半乳糖苷酶β(GLβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY11749Rhesus monkey (Simian)
前動力蛋白受體1(PKR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prokineticin Receptor 1 (PKR1)48T/96TY11750Bos taurus; Bovine (Cattle)
前動力蛋白受體1(PKR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prokineticin Receptor 1 (PKR1)48T/96TY11751Rattus norvegicus (Rat)
親環素B(CYPB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11752Bos taurus; Bovine (Cattle)
親環素B(CYPB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11753Chicken (Gallus)
親環素B(CYPB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11754Mus musculus (Mouse)
親環素B(CYPB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11755Rattus norvegicus (Rat)
親環素B(CYPB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY11756Oryctolagus cuniculus (Rabbit)
緩激肽(BK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Bradykinin (BK)48T/96TY11757Oryctolagus cuniculus (Rabbit)
維生素A(VA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vitamin A (VA)48T/96TY11758General
硫氧化還原蛋白(Trx)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thioredoxin (Trx)48T/96TY11759Spinacia oleracea (Spinach)
抑肽酶(AP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aprotinin (AP)48T/96TY11760Sus scrofa; Porcine (Pig)
成纖維細胞生長因子10(FGF10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 10 (FGF10)48T/96TY11761Homo sapiens (Human)
凝血酶原片段F1+2(F1+2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prothrombin Fragment 1+2 (F1+2)48T/96TY11762Mus musculus (Mouse)
Ⅰ型前膠原(PCⅠ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Procollagen I (PCI)48T/96TY11763Mus musculus (Mouse)
WAP四二硫化物核心域蛋白1(WFDC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for WAP Four Disulfide Core Domain Protein 1 (WFDC1)48T/96TY11764Rattus norvegicus (Rat)
補體因子D(CFD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Complement Factor D (CFD)48T/96TY11765Mus musculus (Mouse)
白介素3(IL3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 3 (IL3)48T/96TY11766Canis familiaris; Canine (Dog)
白介素3(IL3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 3 (IL3)48T/96TY11767Capra hircus; Caprine (Goat)
白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 4 (IL4)48T/96TY11768Capra hircus; Caprine (Goat)
白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 4 (IL4)48T/96TY11769Equus caballus; Equine (Horse)
白介素5(IL5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11770Bos taurus; Bovine (Cattle)
白介素5(IL5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11771Canis familiaris; Canine (Dog)
白介素5(IL5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11772Capra hircus; Caprine (Goat)
白介素5(IL5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11773Equus caballus; Equine (Horse)
白介素5(IL5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11774Chicken (Gallus)
膜聯蛋白A5(ANXA5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Annexin A5 (ANXA5)48T/96TY11775Bos taurus; Bovine (Cattle)
膜聯蛋白A5(ANXA5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Annexin A5 (ANXA5)48T/96TY11776Chicken (Gallus)
膜聯蛋白A5(ANXA5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Annexin A5 (ANXA5)48T/96TY11777Rhesus monkey (Simian)
載脂蛋白C1(APOC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein C1 (APOC1)48T/96TY11778Canis familiaris; Canine (Dog)
載脂蛋白C1(APOC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein C1 (APOC1)48T/96TY11779Rattus norvegicus (Rat)
載脂蛋白C1(APOC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein C1 (APOC1)48T/96TY11780Oryctolagus cuniculus (Rabbit)
線粒體肌酸激酶1A(CKMT1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Creatine Kinase, Mitochondrial 1A (CKMT1A)48T/96TY11781Bos taurus; Bovine (Cattle)
線粒體肌酸激酶1A(CKMT1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Creatine Kinase, Mitochondrial 1A (CKMT1A)48T/96TY11782Chicken (Gallus)
線粒體肌酸激酶1A(CKMT1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Creatine Kinase, Mitochondrial 1A (CKMT1A)48T/96TY11783Sus scrofa; Porcine (Pig)
轉鐵蛋白受體2(TFR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transferrin Receptor 2 (TFR2)48T/96TY11784Mus musculus (Mouse)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。